Figure 1.

Release of AA mediated by increasing concentrations of peroxynitrite in the absence or presence of L-NAME/tB-OOH. [³H]-AA-labelled cells were exposed for 5 min to 1 mM L-NAME and then treated for a further 10 min with 200 μM tBOOH in the absence or presence of increasing concentrations of peroxynitrite (open circles). The open square indicates the [³H]-AA release measured in cells treated with tB-OOH alone. Also shown are the results obtained in cells exposed for 10 min to authentic (closed circles) or decomposed (closed squares) peroxynitrite in the absence of additional treatments. After treatments, the [³H]-AA release was quantified as described in the Methods section. Each point is the mean±s.e.mean of 3–5 separate experiments, each performed in duplicate. ^*P<0.05, ^**P<0.01 vs untreated or tB-OOH/L-NAME-treated cells (ANOVA followed by Dunnet's test).