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Binding of CGRP (a) or Adrenomedullin (b) to Transfected 293 Cells. Cells were grown in 24 well plates and transfected with DNA as indicated below. After 24 h, binding assays for either CGRP (a) or adrenomedullin (b) were performed in triplicate either in the absence (first column, total binding) or presence (second column, non specific binding) of unlabelled ligand and results are shown±s.e.m. Transfected DNAs are indicated on the figures with R1 indicating RAMP-1 etc.
