Figure 1.

Effect of ACh and GTN (1 μM each) on eNOS activity in BAECs as measured by the conversion of [³H]-L-arginine into [³H]-citrulline. The basal activity of eNOS was 4.11±0.6 pmol mg protein⁻¹ min⁻¹. Treatment with L-NMMA (10⁻³ M) reduced basal ³H-citrulline formation by 71.7±5.4%. This treatment reduced the effects of ACh and GTN by over 90%. Data are presented as a mean±s.e.mean of six experiments run in duplicates. ^*Indicates a significant difference from the baseline value.