Figure 2.

(a) Dose response of troglitazone on dexamethasone treated adipocytes. 3T3 adipocytes were incubated with 100 nM dexamethasone for 2 days. After 2 days troglitazone 0–10 μM and dexamethasone, 100 nM were added and incubated for 4 days. At the end of 6 days, cells were removed and glycogen synthesis from ¹⁴C-glucose was assayed in the presence of 100 nM insulin. Data are mean±s.e.mean of n=4, ^**P<0.02, ^***P<0.001 compared to dexamethasone-treated adipocytes. (b) Effect of troglitazone and metformin on dexamethasone-treated adipocytes. 3T3 adipocytes were incubated with or without 100 nM dexamethasone for 2 days. After 2 days troglitazone (1.0 μM) or metformin (1.0 mM) was added to dishes incubated with dexamethasone along with 100 nM dexamethasone for the next 4 days. At the end of 6 days, the dishes were removed and glycogen synthesis was studied in the presence of 100 nM insulin. Data are mean±s.e.mean, n=4, ^***P<0.001 compared to dexamethasone-treated cells. (c) Effect of troglitazone and metformin incubated simultaneously with dexamethasone. 3T3 adipocytes were incubated with troglitazone (1.0 μM) or metformin (1.0 mM) or vehicle along with 100 nM dexamethasone for 6 days. At the end of 6 days glycogen synthesis was studied in the presence of 100 nM insulin. Data are mean±s.e.mean, n=8 for troglitazone and n=6 for other groups. ^**P<0.01 compared to dexamethasone-treated cells.