Effects of JTT-501 and troglitazone on serum or urinary calcium, serum osteocalcin, and urinary DPD in ZDF rats. Using serum and urine samples at 13–15 weeks administration, serum calcium (A), urinary calcium (B), serum osteocalcin (C), and urinary DPD (D) were measured as described in Methods. Each column represents the mean±s.e.mean (n=8). ¶P<0.05, ¶¶P<0.01 vs lean (Student's t-test). *P<0.05, **P<0.01 vs ZDF control (ANOVA followed by Dunnett's test). †P<0.05, ††P<0.01 vs ZDF control (Student's t-test).