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Individual experimental traces (a) and mean data (b) showing the increase in tone obtained when the inhibitor of nitric oxide synthase, L-NAME (100 μM), was added to endothelium-containing (E+) and endothelium-denuded (E−) rings of porcine cerebral artery in the absence of a vasoconstrictor. Each point is the mean±s.e.mean of six observations. ***P<0.001 indicates that L-NAME had a significant effect on tone.
