Figure 2.

Scatchard analysis of the effect of betaxolol on the specific binding of [³H]-BTX-B to rat cortical membranes. Membranes were incubated in the presence or absence of 10 μM betaxolol as described in Methods. The results represent data from a single typical experiment performed in duplicate. Values for B_max were 1.46 pmol mg⁻¹ protein and 1.24 pmol mg⁻¹ protein in the absence and presence of betaxolol, respectively. The K_D values were 29.2 and 55.8 nM, respectively. Two additional experiments yielded similar results.