Figure 4.

Inhibition of F0F1-ATPase of digitonin-solubilized brain mitochondrial preparation by resveratrol (7 μM) and genistein (50 μM) at several concentrations of 0.1–4 mM ATP as shown by Lineweaver–Burk plots. The linear correlation coefficients for all three groups are between 0.996 and 0.999. Note the different V_max among the control, resveratrol and genistein, and the change in K_m induced by resveratrol. The control F0F1-ATPase activity of the preparation at 4 mM ATP was 0.797 (0.007) μmol ATP hydrolysed min⁻¹ (mg protein)⁻¹ from three trials performed before, during and after the experimental trials.