Figure 1.

A recording of [Ca²⁺]_i converted from the fura-2-fluorescence ratio (F340/F380). The trace of fluorescence ratio was converted onto the linear scale of the absolute value of [Ca²⁺]_i as described in Methods. The [Ca²⁺]_i (ordinate) is shown in nM. The peak [Ca²⁺]_i is determined as indicated by an arrow. The shaded area indicates the area under the [Ca²⁺]_i curve (integrated [Ca²⁺]_i elevation).