Figure 8.

Effects of YD-3 on thrombin-induced aggregation of platelets pretreated with chymotrypsin. Human platelets were incubated with chymotrypsin (10 u ml⁻¹) for 30 min at 37°C before rewashing, as described under Methods. Chymotrypsin-treated platelets were incubated with DMSO (0.5%, control) or YD-3 (50 μM) at 37°C for 3 min, platelet aggregation was then induced by the addition of thrombin (T, 0.1 u ml⁻¹) and/or U46619 (U, 2 μM).