Figure 6.

The effect of inhibitors of phospholipase C and protein kinase C on the CAT gene transcription induced by corticosterone (1 μM, 2 h) in LMCAT cells. The phospholipase C inhibitor (U-107) and the protein kinase C inhibitor (tamoxifen) were used at the indicated concentrations, alone or with imipramine (10 μM), for 3 days. Corticosterone (1 μM) was added 2 h before harvesting the cells for an assay of CAT enzyme activity (expressed as a percentage of the control that received corticosterone alone). The data are presented as mean±s.e.mean, and the significance of differences between the means was evaluated by the Dunnett's test following a two-way analysis of variance (^**P<0.001 vs control group; ⁺⁺P<0.001 vs imipramine group).