Figure 8.

The effect of protein kinase activators on the CAT gene transcription induced by corticosterone (1 μM, 2 h) in LMCAT cells. An activator of protein kinase C (TPA- phorbol 12-myristate 13-acetate) and an activator of protein kinase A (For-forskolin) were used at the indicated concentrations, alone or with imipramine (10 μM), for 3 days. Corticosterone (1 μM) was added 2 h before harvesting the cells for an assay of CAT enzyme activity (expressed as a percentage of the control that received corticosterone alone). The data are presented as mean±s.e.mean, and the significance of differences between the means was evaluated by the Dunnett's test following a two-way analysis of variance (^*P<0.05 vs respective imipramine group).