Figure 9.

The effects of some modulators on the CAT gene transcription induced by corticosterone (1 μM, 2 h) in LMCAT cells. Imipramine at a concentration of 10 μM was added to the indicated cultures for 3 days. An inhibitor of protein kinase A (cyclic AMPS), an inhibitor of protein kinase G (cyclic GMPS), a calcium/calmodulin kinase inhibitor (W-13), an activator of protein kinase C (TPA), an activator of protein kinase A (For-forskolin), an inhibitor of protein kinase C (Tam-tamoxifen), an inhibitor of phospholipase C (U-107) and an L-type Ca²⁺ channel blocker (Nif-nifedipine) were used at the indicated concentrations for 4 h. Corticosterone (1 μM) was added 2 h before harvesting the cells for an assay of CAT enzyme activity (expressed as a percentage of the control that received corticosterone alone). The data are presented as mean±s.e.mean, and the significance of differences between the means was evaluated by the Dunnett's test following a two-way analysis of variance (^*P<0.05; ^**P<0.001 vs respective control group; ⁺⁺P<0.001 vs respective imipramine group).