Figure 4.
Effects of anti-CD3/CD28 stimulated PBMC culture supernatants in the presence of drugs on ICAM-1 and VCAM-1 expression of HUVEC. PBMC culture supernatants stimulated with anti-CD3/CD28 in the presence of various concentrations of FK506, CsA, DEX and MTX as shown in Figure 2 were added to HUVEC monolayers. After 16 h culture, the expression of ICAM-1 and VCAM-1 on the HUVEC was determined by Cell ELISA. The data are the mean of percentage inhibition of five individual PBMC culture supernatants.