Figure 4.

Chymostatin abrogated eosinophil suppression of PMNC proliferation in response to PHA in the separated culture system. PMNC (5×10⁵ cells ml⁻¹) were incubated with PHA-p (2 μg ml⁻¹) for 72 h in the presence or absence of autologous eosinophils (1×10⁵ cells ml⁻¹) with or without chymostatin (CH) at 1×10⁻⁶ M in the coculture or separated culture systems. The cultures were pulsed with 1 μCi of [³H]-thymidine for the final 8 h and the incorporation of [³H]-thymidine was assayed using a liquid scintillation counter. [³H]-thymidine incorporation was expressed as counts per minute (c.p.m.). (n=8).