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OA enhances NF-κB DNA binding. Electrophoretic mobility shift assay for the transcription factor NF-κB was performed after treatment of TF-1 cells with medium, OA (30 ng ml−1, 6 h), or SB203580 (30 min pretreatment) at 1, 5 and 10 μM plus OA (6 h). Band shift assay was performed as described in ‘Methods' utilizing 32P-labelled oligonucleotide specific for NF-κB. Excess of free probe is shown.
