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SB203580 at 1 μM inhibits NF-IL6 DNA binding activity. Electrophoretic mobility shift assay for the transcription factor NF-IL6 was performed after treatment of TF-1 cells with medium, OA (30 ng ml−1, 6 h), and SB203580 at 1 μM (30 min pretreatment) plus OA. Band shift assay was performed as described in ‘Methods' utilizing 32P-labelled oligonucleotide specific for NF-IL6.
