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. 2000 Sep;131(2):231–238. doi: 10.1038/sj.bjp.0703572

Figure 2.

Figure 2

RT–PCR assay of the expression of mRNA for endothelial nitric oxide synthase (eNOS) in control, diabetic, and pravastatin-treated diabetic rat aortae. (A) Expression of mRNA for eNOS as assayed by RT–PCR. (B) Quantitative analysis of expression of mRNA for eNOS by scanning densitometry. Control rats (n=6, open column); STZ-induced diabetic rats (n=6, closed column); pravastatin-treated diabetic rats (n=6, stippled column). Values are mean±s.e.mean of six determinations (eNOS/GAPDH ratios). The RT–PCR assay was performed as described in Methods. Each total RNA preparation (1.0 mg) was reverse-transcribed, and half of the cDNA product was PCR-amplified, using various primers, for 20 or 28 cycles. A portion of the PCR reaction product was electrophoresed on a 1.5% agarose gel containing ethidium bromide.