Figure 7.

Time course of the activation of ERK1, p38MAPK and JNK1. A549 cells were treated for the times indicated by either 10 nM EGF or 1 ng ml⁻¹ IL-1β. Immunoprecipitates of the selected MAPK's were examined for activation status by Western blotting with antibodies to either phosphothreonine (PT) or phosphotyrosine (PY). EGF induced a 20 fold activation of ERK1 from 5 min, a 10-fold activation of p38MAPK from 10 min and a 2 fold activation of JNK1 from 5 min. Whereas, IL-1β induced a 5 fold activation of ERK1 at 1 h, a 10 fold activation of p38MAPK at 1 h and a 30 fold activation of JNK1 at 1 h.