Figure 2.

Increased receptor density in alpha-1a AR mutant by treatment with prazosin but not with KMD-3213 in time- (A) and concentration- (B) dependent manner. (A) CHO cells expressing the alpha-1a AR mutant were treated with 100 nM prazosin or KMD-3213 for the time indicated and the specific binding of [³H]-prazosin were measured as outlined under Methods. (B) CHO cells expressing the mutant alpha-1a AR was treated for 72 h with various concentrations of prazosin or KMD-3213 and the specific binding of [³H]-prazosin was measured. Data represent mean±s.e.mean from three experiments.