Figure 5.

Effects of IBMX on the 100 μM levcromakalim-induced current and hyperpolarization in a conventional whole-cell recording. (a) Although no membrane current was evoked by application of IBMX 100 μM, subsequent application of 100 μM levcromakalim caused a large inward current at −50 mV. Bath solution 140 mM K⁺ PSS, pipette solution 140 mM K⁺ containing 5 mM EGTA. The dashed lines indicate the zero current level. (b) 100 μM IBMX had no effect on the 100 μM levcromakalim-induced membrane hyperpolarization in current-clamp mode. Bath solution 5 mM K⁺ PSS, pipette solution 140 mM K⁺ containing 5 mM EGTA.