Figure 4.

Effects of MT-7 on atropine-induced [³H]-NMS dissociation from M₁ receptors. CHO/M₁ cell membranes were incubated at 30°C first with 1 nM [³H]-NMS to equilibrium for 60 min and then with either vehicle (control) or 3 nM MT-7 for 20 min. The dissociation [³H]-NMS was started by the addition of 10 μM atropine and the incubation was stopped at the indicated time points. [³H]-NMS binding data are expressed as ln B/Be, where B is the amount of [³H]-NMS specifically bound at the indicated time and Be is the specific binding determined at zero time. Lines represent the least-squares linear regressions of the data. Values are the mean of three experiments.