Figure 1.

CS-2170 induces apoptosis in haematopoietic cancer cell lines. (A) Dose-response of the effect of CS-2170 on cell viability. Haematopoietic cancer cells (Jurkat, NSO, HL-60 and Ramos) and nonmalignant cell lines (MDCK and NIH-3T3) were incubated for 4 h with 1–10 μg ml⁻¹ of CS-2170. Cell viability was determined by the MTT assay as described in Methods and it is expressed as a percentage with respect to control cells. (B) DNA fragmentation induced by CS-2170 was observed in the agarose gel electrophoresis as described in Methods. Jurkat, NSO, HL-60, Ramos, MDCK and NIH-3T3 cells were untreated (−) or incubated for 16 h with 10 μg ml⁻¹ of CS-2170 (+). (C) Fluorescence microscopic analysis of Jurkat, HL-60 and NIH-3T3 nuclei with Hoechst 33342 staining. Cells were untreated (-CS-2170) or treated with 5 μg ml⁻¹ of CS-2170 for 6 h (bar=10 μm).