Figure 2.

Involvement of caspases in induction of phosphatidylserine externalization by CS-2170. A representative annexin-V/IP assay with Jurkat is shown. This assay reveals the early phosphatidylserine translocation to the surface of apoptotic cells by incubating the cells with FITC-labelled annexin V and the latest stages of apoptotic or necrotic processes were detected with the vital dye propidium iodide (PI). Cells staining were analysed using the Becton Dickinson FACS Calibur flow cytometer as described in Methods. (A) Control cells. (B, C) Cells were incubated for 4 h with 5 and 10 μg ml⁻¹ of CS-2170 respectively. (D) Cells were incubated with 50 μM Z-VAD.fmk. (E, F). Cells were incubated with 5 and 10 μg ml⁻¹ of CS-2170 respectively in the presence of 50 μM Z-VAD.fmk.