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Effect of L-NAME on ACh-induced relaxation. Cumulative dose-response curves of ACh either in control conditions or after 30 min preincubation with 100 μM L-NAME were performed on 10−7 M phenylephrine-precontracted rat thoracic aortic strips stretched at different resting tensions (A: 0.7 g and B: 2.5 g). The contraction obtained after phenylephrine is set as 100%. Values are the mean±s.e.mean of at least four experiments.
