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Effect of ACh on PGF2α-precontracted rat aortic strips. Cumulative dose-response curves of ACh were performed in control conditions (A and B) and in the presence of 5 μM indomethacin (C and D). Aortic strips were stretched at a resting tension of 0.7 g (A and C) and 2.5 g (B and D) and precontracted with 10−5 M PGF2α (PGF2α). Black squares indicate ACh (10−7–3×10−7–10−6 M) administration. Typical traces are shown. W=washing.
