Figure 4.

Effect of SIB-1893 on NMDA-evoked whole cell current. (A). Representative traces of NMDA-evoked whole cell currents in the absence and presence of SIB-1893. Whole cell currents were recorded from cultured cortical neurones at 7–10 DIV, with voltage clamped at −60 mV in Mg²⁺ free solution. SIB-1893 (20 or 200 μM) was delivered to the cell 30 s prior to delivery of 50 μM NMDA. (B) A summary of the effect of SIB-1893 on NMDA-evoked current. Both peak and steady state responses were recorded. At a concentration of 20 μM, SIB-1893 significantly reduced peak NMDA current to 55.9±6.5% and reduced the steady state current to 48.4±4.3% of control values (n=5, ^*P<0.05). At a concentration of 200 μM, SIB-1893 significantly reduced both peak and steady state NMDA-evoked current to 36.6±3.7 and 31.3±5.0% of control values respectively (n=5, ^*P<0.05).