Figure 6.

Effect of 9-cRA on the protein expression of osteopontin, fibronectin, VCAM-1 and ICAM-1 in human mesangial cells. Confluent human mesangial cells were incubated up to 24 h in 10% FCS with 1 μM 9-cRA and total protein was extracted. Twenty μg of every protein extract was electrophoresed and transferred onto nitrocellulose. Membranes were probed with primary antibodies against OP, FN, VCAM and ICAM-1 and blots were developed using secondary antibodies conjugated with horseradish peroxidase for viewing enhanced chemiluminescence as described in Methods.