Figure 7.

Expression of mRNA for RARs and RXRs and its modulation by 9-cRA. Quiescent, confluent human mesangial cells were incubated up to 8 h with 1 μM 9-cRA and total RNA was extracted. Northern blot for the subtypes α, β and γ of either RAR or RXR was carried out as described in Methods. The blots were stripped and rehybridized with a cDNA probe for the housekeeping gene GAPDH to adjust for small variations in RNA loading and transfer to the filter. The autoradiograms shown are representative of three separate experiments. A second RXRγ autoradiogram with lower exposition is also shown (centre right) in order to improve the observation of the two bands between 28S and 18S rRNA.