Figure 5.

Effect of nifedipine on the phosphorylation and protein level of ERK 1/2 (p44/42) in rat VSMC. (A) The phosphorylation or protein level of ERK 1/2 was detected by immunoblot analysis using antibody phosphospecific or specific for ERK 1/2, as described in Methods. p-ERK 1/2 or ERK 1/2 shows the phosphorylation or the protein level of ERK 1/2, respectively. (B) The quantitation of the phosphorylated ERK 1/2 from three separate experiments is shown, and each bar represents the mean±s.e.mean. ^*P<0.05, ^**P<0.01 vs control.