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. 2000 Dec;131(8):1521–1530. doi: 10.1038/sj.bjp.0703730

Figure 6.

Figure 6

Effect of nifedipine on the phosphorylation and protein level of MEK 1/2 in rat VSMC. (A) The phosphorylation or protein level of MEK 1/2 was detected by immunoblot analysis using antibody phosphospecific or specific for MEK 1/2, as described in Methods. p-MEK 1/2 or MEK 1/2 shows the phosphorylation or the protein level of MEK 1/2, respectively. (B) The quantitation of the phosphorylated MEK 1/2 from three separate experiments is shown, and each bar represents the mean±s.e.mean. *P<0.05, **P<0.01 vs control.