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. 2000 Dec;131(8):1651–1658. doi: 10.1038/sj.bjp.0703743

Figure 3.

Figure 3

Simultaneously measured changes in [Ca2+]i in ISC. Cells grown to confluence on culture membranes were loaded with Fura-2 and membranes bearing these cells and mounted in a miniature Ussing chamber attached to the stage of an inverted microscope so that changes in [Ca2+]i (upper panel) and ISC (lower panel) could be measured simultaneously. The figure shows the responses evoked by a series of 30 s pulses of increasing concentrations (1–500 μM) of ATP that were delivered to the cells' apical membrane as denoted by the arrows.