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[Ca2+]i-independent responses to ATP. Fura-2-loaded cells on culture membranes mounted in the miniature Ussing chamber that changes in [Ca2+]i (upper panel) and ISC (lower panel) could be measured simultaneously. The cells were first exposed to 1 μM ionomycin and then stimulated with a pulse of 100 μM in the continued presence of this ionophore. Essentially identical responses were obtained in 22 experiments.
