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Expression of P2Y receptor mRNA. (a) PCR products obtained using RNA isolated from cells grown on glass Petri dishes. (b) Products obtained RNA isolated from age-matched cells at identical passage grown on Transwell membranes. For each set of primers, products obtained under standard conditions are in lane i, whilst the results of the ‘no RT' and negative (i.e. water) controls are in lanes ii and iii respectively. Essentially identical data obtained in three independent analyses.
