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Effect of removal of calcium ions from (A) and increasing potassium ion concentration (100 mM) in (B) the perfusing aCSF on the 5-HT overflow from dialysate from the SCN region at ZT 6. Calcium ions were removed from, or potassium ions were added to, the aCSF for a period of 60 min, starting at time 0. n=3; *P<0.05; **P<0.01, ***P<0.001 one way ANOVA with repeated measures with Student Newman Keuls post hoc test).
