Figure 6.

Effect of 10 μM neocuproine on the relaxations induced by (A) 1 Hz electrical field stimulation (EFS) and (B) 1 μM NO (added as acidified NaNO₂) in control conditions and after treatment of the muscle strips with DETCA (0.5 mM) or 3-amino-1,2,4-triazole (Atr, 1 mM). Results are expressed as percentage enhancement induced by neocuproine of the control relaxation, which was taken as 100%, in respective conditions (saline, DETCA or 3-amino-1,2,4-triazole). Results are shown as mean±s.e.mean for n=8 – 12 experiments. ^*P<0.05 is considered as significantly different from respective controls (paired Student's t-test) and #P<0.05 is considered as significantly different from the effect of neocuproine in untreated (saline) muscle strips (one way ANOVA followed by post hoc Dunnett's test, NS=not significant).