Figure 6.

Effect of glibenclamide on CF efflux from isolated rat hepatocytes CF-loaded isolated rat hepatocytes were incubated in CF diacetate-free medium in the presence or absence of 50 μM glibenclamide, 500 μM tolbutamide or 5 mM probenecid for 90 min. Intracellular retained CF was then determined by fluorimetry and expressed as per cent of initial dye staining. The values are the mean±s.d.mean of at least three independent experiments in triplicate. ^*P<0.05 when compared to cells not treated by glibenclamide.