Figure 5.
Percentage of nuclei staining positive for TUNEL in sham-operated control hearts or the rabbit hearts exposed to I/R receiving either vehicle or 3 μg kg−1 benidipine. Four longitudinal sections from each heart were cut and embedded in a paraffin block, and two slides per block were evaluated histologically. For each slide, a total of 10 fields were randomly chosen, a total of 100 cells per field were counted, and the percentage of myocytes exhibiting positive staining apoptosis was determined. **P<0.01 vs vehicle group.