Figure 3.

Effects of NS-1619 on membrane current and [Ca²⁺]_i. (A) Membrane current was measured under voltage-clamp at a holding potential of −40 mV. [Ca²⁺]_i was simultaneously measured in the same manner as in Figure 2. Application of 30 μM NS-1619 induced an outward current and increased [Ca²⁺]_i. (B) The amplitude of the sustained component of outward current induced by 30 μM NS-1619 at holding potential of −40 mV was measured in seven myocytes. (C) The [Ca²⁺]_i before and during the application and after washout of 30 μM NS-1619 under voltage-clamp at −40 mV were summarized from the results obtained in the same cells shown in (B). The statistical significance of the difference vs the control group is indicated by ^**P<0.01.