Figure 5.

Effects of the removal of extracellular Ca²⁺ on NS-1619-induced responses of membrane potential (M.P.) and [Ca²⁺]_i. The 2.2 mM Ca²⁺ in the external solution was replaced by 0.5 mM Cd²⁺. (A) The change in M.P. and that in [Ca²⁺]_i as the average from whole cell area induced by application of 30 μM NS-1619 in the absence of external Ca²⁺. (B) and (C) Summarized results of the changes in M.P. (B) and [Ca²⁺]_i (C) by 30 μM NS-1619 under normal and Ca²⁺-free conditions are illustrated. The number of experiments is given in parentheses. The statistical significance of the difference vs control is indicated by ^**P<0.01.