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Recordings of the effects of carbachol (50 μM), thapsigargin (Tg; 100 nM) and sodium nitroprusside (SNP; 10 μM) on cytoplasmic calcium levels (measured as the Fura-2 ratio; R340/380) in single smooth muscle cells of the mouse anococcygeus. Each trace comprises the integrated data from (a) 6 cells, (b) 12 cells, and (c) 9 cells.
