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. 2001 Mar;132(5):1035–1046. doi: 10.1038/sj.bjp.0703899

Figure 4.

Figure 4

Influences of ovariectomy and oestrus cycle on EDHF-mediated relaxation induced by ACh in rat mesenteric arteries. (a) Typical traces illustrating the relaxant responses to ACh during 300 nM Phe-induced contraction in control (sham-operated) rat mesenteric arteries in the presence of 10 μM indomethacin and 100 μM L-NOARG. Right panel shows that the remaining relaxation to ACh was abolished by further treatment with 500 nM apamin and 100 nM ChTX. Indomethacin, L-NOARG, apamin and ChTX were added to the bath 15–20 min before application of Phe. (b) Concentration-response curves for ACh-induced relaxation in mesenteric arteries from rats that were sham-operated, ovariectomized, and ovariectomized and 17β-oesradiol-treated. (c) Concentration-response curves for ACh-induced relaxation in mesenteric arteries from oestrus and diestrus rats. All curves were constructed in the presence of indomethacin and L-NOARG. The points are means of 6–12 experiments; vertical lines show s.e.mean. Responses are expressed as per cent relaxation of Phe-induced contraction.