Figure 1.

Effects of treatment with A23187, TG, and DBHQ on [³H]-STX binding capacity in adrenal chromaffin cells. (A) Cells were treated for up to 96 h with DMSO, 1 μM A23187, 100 nM TG, 10 μM or 100 μM DBHQ, then washed with KRP buffer, and tested for [³H]-STX binding assay. In a parallel experiment, cells exposed for 48 h to 1 μM A23187 or 100 nM TG were washed with culture medium (indicated by arrow), then incubated without A23187 or TG, and subjected to [³H]-STX binding assay at 72 and 96 h. Mean±s.e.mean (n=5). ^*P<0.05, significant decrease by A23187 or TG compared to DMSO-treated cells. (B) Cells were treated for 48 h with DMSO, A23187 or TG at indicated concentrations, and tested for [³H]-STX binding ability. Mean±s.e.mean (n=5). ^*P<0.05, significant decrease by A23187 or TG. (C) Scatchard plot of [³H]-STX binding to cells treated with DMSO, 1 μM A23187 or 100 nM TG for 48 h. Data are representative of one experiment from three independent experiments with similar results.