Figure 3.

Effects of (a) high-K⁺ (60 mM), PSS, (b) ouabain (10 and 100 μM), (c) charybdotoxin (ChTX, 100 nM) and charybdotoxin plus apamin (1 μM), and (d) Ba²⁺ (50 μM) or 18α-glycyrrhetinic acid (18α-GA, 50 μM) on acetylcholine (ACh)-induced relaxations in the presence of 100 μM L-NAME. Tissues were precontracted with phenylephrine (1 μM) except for those in high-K⁺ PSS experiments in which tissues were contracted with high-K⁺ PSS. Relaxations are presented as percentage reductions of the precontractions. Data are mean±s.e.mean. (n=3 – 8). ^*P<0.05 vs control, one-way ANOVA followed by Tukey's test.