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Effects of different doses of ouabain (top), digoxin (centre), or digitoxin (bottom) on the release of pregnenolone in the presence (solid columns) or absence (hatch columns) of 25-OH-cholesterol (1×10−6 M). **P<0.01 compared with the value at drug=0 M. ++P<0.01 compared with vehicle-treated group. Each column represents mean±s.e.mean.
