Figure 6.

Effects of ouabain, digoxin, and digitoxin on the protein expression of cytochrome P450scc (top) and StAR (bottom) protein in rat granulosa cells. Rat granulosa cells were incubated with ouabain (10⁻⁵ M), digoxin (10⁻⁵ M), or digitoxin (10⁻⁵ M) at 37°C for 2 h. Then, the cells were collected and analysed by Western blotting. Each lane was loaded with 10 μg protein of sample. ^*P<0.05 compared with the value at drug=0 M. Each column represents mean±s.e.mean.