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The mRNA expression of PACAP/VIP family receptors in neonatal rat spinal cord using RT – PCR. Total RNA was isolated by the guanidinium isothiocyanate/CsCl procedure from neonatal rat spinal cord (1-day-old). RT – PCR was performed using RNA PVR Kit (TaKaRa, Ohtsu, Japan) as described in ‘Methods'.
