Figure 4.
Effect of LPS (10 μg ml−1) on the antiaggregatory responses of 8-iso PGF2α (10−6 M) in U46619 (5×10−7 M) stimulated whole blood. Responses are shown in untreated blood after (a) collection without storage or (b) after storage for 6 h. For comparison, responses are shown in (c) in blood stimulated with LPS for 6 h. The data is represented as percentage of control response obtained in each condition in blood stimulated with U46619 alone. Each data point comprises data from n of five separate donors. Experiments illustrated in (a), (b) or (c) were performed in parallel. *Illustrates a significant difference between aggregation in comparison to control response in non-LPS-treated blood using two-way ANOVA. A P value of less than 0.05 was taken to be significant.