Figure 2.

Effect of DRG cell density on responses to activators of adenylyl cyclase. DRG cells were plated at 500, 5000, 10,000 and 15,000 cells well⁻¹, and [³H]-cyclic AMP production assayed in response to iloprost (10 μM), PGE₁ (10 μM), PGE₂ (10 μM) and forskolin (50 nM and 10 μM). Basal [³H]-cyclic AMP production ([cAMP]/[total AXP]×1000) for each condition was: 500 cells, 2.00±0.19^*; 5000 cells, 3.51±0.35; 10,000 cells, 3.46±0.39 and 15,000 cells, 3.01±0.26. ^*P<0.05 compared with 5000 and 10,000 cells well⁻¹ groups, one-way ANOVA with Bonferroni's post-tests. Data represent mean±s.e.mean of 3 – 6 experiments, each performed in triplicate.