Figure 5.

Cyclic AMP accumulation in CHO cells expressing NPFFR. (A) Cells pretreated or not with PTX were incubated with vehicle or 1 μM SQA-neuropeptide FF in the presence or absence of 10 μM forskolin as described in the Methods section. Each value represents the mean±s.e.mean for triplicate determinations. The data are representative of three independent experiments. (B) Cells were incubated in the presence of the various concentrations of SQA-neuropeptide FF and 10 μM forskolin as described. Each point represents the mean±s.e.mean of triplicate determinations.